Comparison of Silica-based Column with Polymer-based Column

Asahipak ODP columns are suitable for the analysis of macromolecules such as proteins. A comparison of resolution between the polymer-based (ODP) and the silica-based (ODS) columns shows that the resolution of the former is better.


Sample :
1. Ribonuclease, 2. Cytochrome c, 3. Lysozyme, 4. Chymotrypsinogen A, 5. Ovalbumin

Columns      : Shodex Asahipak ODP-50 6D, ODS (6.0 mm I.D. x 150 mm each)
Eluent       : 0.05 % TFA aq./CH3CN
               Linear gradient: 0 to 30 min, 90/10 to 40/60
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C

Sample Name Index

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

Product Name Index

Applications