Omega-3 fatty acids are one type of the unsaturated fatty acids. They are “essential fatty acids” that humans must ingest them through foods since humans cannot synthesize them.
According to the United States Pharmacopeia and the National Formulary (USP 43-NF 38*), analysis of oligomers, impurities, in “Omega-3 Free Fatty Acids” should be carried out with three columns (connected in series) filled with L21 packing material and meets following requirements. The combination of GPC KF-802.5, KF-802 and KF-801 confirmed the requirements were met.

System suitability requirements:
   Resolution of monodocosahexaenoin and didocosahexaenoin: ≥ 2.0
   Resolution of didocosahexaenoin and tridocosahexaenoin: ≥ 1.0

*The version at the time of the application acquisition.


Sample : 40 µL
1. Tridocosahexaenoin  0.2 mg/mL
2. Didocosahexaenoin  0.3 mg/mL
3. Monodocosahexaenoin  0.5 mg/mL

Columns      : Shodex GPC KF-802.5 + KF-802 + KF-801 (8.0 mm I.D. x 300 mm each)
Eluent       : THF
Flow rate    : 0.8 mL/min
Detector     : RI
Column temp. : 25 °C