Assay of Polyethylene Glycol 3350 According to USP-NF Method (SB-802.5 HQ)

According to the United States Pharmacopeia and the National Formulary (USP 40-NF 35*), analysis for the assay of polyethylene glycol 3350 should be carried out with a column filled with L25 packing material and meets following requirement. The OHpak SB-802.5 HQ confirmed the requirement was met.

System suitability requirement:
 Relative standard deviation (RSD): ≤ 1.5%

*The version at the time of the application acquisition. 


Sample : 20 μL
Polyethylene glycol 3350
20 mg/mL in eluent


Columns      : Shodex OHpak SB-G 6B (6.0 mm I.D. x 50 mm) 
               + SB-802.5 HQ (8.0 mm I.D. x 300 mm)
Eluent       : 50 µg/mL of NaN3 aq.
Flow rate    : 0.8 mL/min
Detector     : RI
Column temp. : 35 °C

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