Analysis of Calcium Acetate Capsules According to USP-NF Method (YK-421)

​Calcium acetate is a food additive often used in European and US countries as preservatives and pH adjusting agents, as well as to enhance the nutritional value of the food products. According to the United States Pharmacopeia and the National Formulary Pharmacopeial Forum (USP 43-NF 38*), "ASSAY" for "Calcium Acetate Capsules" should be carried out with a column filled with L76 packing material. The IC YK-421, a column filled with L76 packing material, confirmed the requirements were met.

System suitability requirementsTheoretical plate number (Standard solution): ≥ 1,000
Relative standard deviation (RSD) (Standard solution): ≤ 2.0%

*The version at the time of the application acquisition.

Sample: 10 µL
(Standard solution) Calcium acetate 0.08 mg/mL in water

  1.  
Column
Shodex IC YK-421 (4.6 mm I.D. x 125 mm)
Eluent
0.75 mM Dipicolinic Acid and 1.7 mM Nitric Acid in water/Acetone=900/100
Flow rate
1.9 mL/min
Detector
Non-suppressed conductivity
Column temp.
35 ℃

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