Epoetin is an erythropoietin preparation used to treat anemia. According to the United States Pharmacopeia and the National Formulary (USP 41-NF 36*), "IMPURITIES: Limit of High Molecular Weight Proteins" for "Epoetin" should be carried out with a column filled with L20 packing material. The PROTEIN LW-803, a column filled with L20 packing material, confirmed the requirements were met.

System suitability requirementsMonomer retention time (System suitability solution): 8 - 10 min
Relative retention times (System suitability solution): 0.9 (dimer) and 1.0 (monomer) 
Relative standard deviation (RSD) of monomer peak area (System suitability solution): < 2.0%

*The version at the time of the application acquisition.

Sample: 40 µL
(System suitability solution) Erythropoietin 2 mg/mL in eluent
**Heated at 80 ℃ for 30 min

1. 1.Dimer
2. 2.Monomer