Mogroside V is a natural sweetener that is included in Momordica Grosvenori, and it is said that the sweetness of Mogroside V is several hundred times of sucrose. Morgroside V in Momordica Grosvenori was analyzed by Asahipak NH2P-50 4E, a polymer-based amino column.

(Pretreatment)
1) Exactly weighting 0.2 g of Momordica Grsvenori, and exactly diluting to 10 ml total with the eluent.
2) Leaving it quietly for 2 hours.
3) Filtering it by a 0.45 um-membrane-filter before injecting into HPLC.



Sample : 20 μL
1. Mogroside V

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : H2O/CH3CN=26/74 
Flow rate    : 1.0 mL/min
Detector     : UV (203 nm)
Column temp. : 40 °C