Saccharides and sweeteners in yogurt (drink type) were analyzed using Asahipak NH2P-40 2D, a polymer-based amino column, with LC/MS/MS detection. Alkaline conditions promote the deprotonation of hydroxyl groups at the time of ionization. Therefore, alkaline conditions are suitable for high sensitive detection of substances with hydroxyl groups such as saccharides under the negative mode.
*Sales of Asahipak NH2P-40 2D used in this application is discontinued. Please consider trying Asahipak NH2P-50 2D.

Sample pretreatment

1. 1.Add 3 mL of CH₃CN to 1 mL of yogurt (drink type) and mix.
2. 2.Centrifuge (3,000 rpm x 5 min)
3. 3.Add 9.9 mL of H₂O/CH₃CN=1/1 to 0.1 mL of the supernatant and mix (= sample solution)

Sample: 5 μL
Pretreated Yogurt (drink type)

1. Aspartame
2. Fructose
3. Glucose
4. Rebaudioside A
5. Sucrose
6. Lactose