Saccharides and sweeteners in yogurt (drink type) were analyzed using Asahipak NH2P-40 2D, a polymer-based amino column, with LC/MS/MS detection. Alkaline conditions promote the deprotonation of hydroxyl groups at the time of ionization. Therefore, alkaline conditions are suitable for high sensitive detection of substances with hydroxyl groups such as saccharides under the negative mode.
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(Pretreatment of sample)
1) Add 3 mL of CH₃CN to 1 mL of yogurt (drink type) and mix.
2) Centrifuge (3,000 rpm x 5 min)
3) Add 9.9 mL of H₂O/CH₃CN=1/1 to 0.1 mL of the supernatant and mix (= sample solution)


Sample : 5 μL
Yogurt (drink type)
Aspartame
Fructose
Glucose
Rebaudioside A
Sucrose
Lactose

Column       : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
Eluent       : (A); 0.1 % NH3 aq./(B); CH3CN
               Isocratic; (B %) 78 %
Flow rate    : 0.2 mL/min
Detector     : ESI-MS (SIM Negative)
Column temp. : 30 °C