Simultaneous Analysis of Sweeteners and Amino Acids in Energy Drink (NH2P-40 2D)

Sweeteners in an energy drink were analyzed using Asahipak NH2P-40 2D, a polymer-based amino column, with LC/MS/MS detection. Under these conditions, sweeteners, amino acids and water-soluble vitamins such as carnitine and pyridoxine can be simultaneously analyzed.
*Sales of the product used in this application is discontinued, however if you are still interested in this application, please contact us.


Sample : 5 μL
500-fold diluted Energy drink with H₂O/CH₃CN=1/1
Sucralose
Acesulfame (k)
meso-Erythritol
Carnitine
Leucine
Isoleucine
Valine
Pyridoxine
Rebaudioside A

Column       : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
Eluent       : (A) 0.1 % NH3 aq./(B) CH3CN
               Isocratic ; (B %) 78 %
Flow rate    : 0.2 mL/min
Detector     : ESI-MS (SIM Positive/Negative)
Column temp. : 30 °C