Sweeteners in an energy drink were analyzed using Asahipak NH2P-40 2D, a polymer-based amino column, with LC/MS/MS detection. Under these conditions, sweeteners, amino acids and water-soluble vitamins such as carnitine and pyridoxine can be simultaneously analyzed.
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Sample : 5 μL
500-fold diluted Energy drink with H2O/CH3CN=1/1
Sucralose
Acesulfame (k)
meso-Erythritol
Carnitine
Leucine
Isoleucine
Valine
Pyridoxine
Rebaudioside A
Column : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
Eluent : (A) 0.1 % NH3 aq./(B) CH3CN
Isocratic ; (B %) 78 %
Flow rate : 0.2 mL/min
Detector : ESI-MS (SIM Positive/Negative)
Column temp. : 30 °C
Sample Name Index
Applications
- Acesulfame K (SC1011)
- Analysis of Acesulfame K and Sucralose (SC1011)
- Sucralose (SP0810)
- Stevioside and Rebaudioside A (NH2P-50 4E)
- LC/MS Analysis of Various Steviol Glycosides (NH2P-40 2D)
- Quantification of Purified Stevia Extract (VG-50 4E)
- Steviol Purity Test of Purified Stevia Extracts According to Japanese Pharmaceutical Excipients Method (C18M 4E)
- Analysis of Mogroside V in Momordica Grosvenori (NH2P-50 4E)
- LC/MS Analysis of Various Sweeteners (NH2P-40 2D)
- Simultaneous Analysis of Saccharides and Sweeteners in Yogurt Drink (NH2P-40 2D)
- Effects of Eluent pH on HILIC Mode (VG-50 2D)
- Analysis of Thaumatin (SP-825)