• Product Search
  • Application Search
  • Distributors
  • Support

LC/MS Analysis of Various Steviol Glycosides (NH2P-40 2D)


Various steviol glycosides which are used as sweetener were analyzed using Asahipak NH2P-40 2D, a polymer-based amino column, with LC/MS/MS detection. Steviolbioside and Rebaudioside B which have carboxyl group can be eluted under alkaline conditions. Furthermore, alkaline conditions promote the deprotonation of hydroxyl groups at the time of ionization. Therefore, alkaline conditions are suitable for high sensitive detection of substances with hydroxyl groups such as saccharides under the negative mode.
*Sales of the product used in this application is discontinued, however if you are still interested in this application, please contact us.

Sample : 5 μL
50 ng/mL each
(in H2O/CH3CN=1/1)
Rebaudioside C
Rebaudioside F
Rebaudioside A
Rebaudioside D
Rebaudioside B

Column       : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
Eluent       : (A); 0.1 % NH3 aq./(B); CH3CN
               Isocratic ; (B %) 78 %
Flow rate    : 0.2 mL/min
Detector     : ESI-MS (SIM Negative)
Column temp. : 30 °C

Contact us