Various sweetners were analyzed using Asahipak NH2P-40 2D, a polymer-based amino column, with LC/MS/MS detection. Anion substances can be eluted under alkaline conditions. Furthermore, alkaline conditions promote the deprotonation of hydroxyl groups at the time of ionization. Therefore, alkaline conditions are suitable for high sensitive detection of substances with hydroxyl groups such as saccharides under the negative mode.
*Sales of Asahipak NH2P-40 2D used in this application is discontinued. Please consider trying Asahipak NH2P-50 2D.
Sample: 5 μL
100 ng/mL each (in H2O/CH3CN=1/3)
- Dulcin
- Sucralose
- Acesulfame
- Aspartame
- Cyclamate
- Saccharin
- Glucose
- Rebaudioside A
- Sucrose
- Column
- ：Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
- Eluent
- ：(A) 0.1 % NH3 aq./(B) CH3CN
Isocratic; (B %) 80 % - Flow rate
- ：0.2 mL/min
- Detector
- ：ESI-MS (SIM Negative)
- Column temp.
- ：30 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Acesulfame K (SC1011)
- Analysis of Acesulfame K and Sucralose (SC1011)
- Sucralose (SP0810)
- Stevioside and Rebaudioside A (NH2P-50 4E)
- LC/MS Analysis of Various Steviol Glycosides (NH2P-40 2D)
- Quantification of Purified Stevia Extract (VG-50 4E)
- Steviol Purity Test of Purified Stevia Extracts According to Japanese Pharmaceutical Excipients Method (C18M 4E)
- Analysis of Mogroside V in Momordica Grosvenori (NH2P-50 4E)
- Simultaneous Analysis of Sweeteners and Amino Acids in Energy Drink (NH2P-40 2D)
- Simultaneous Analysis of Saccharides and Sweeteners in Yogurt Drink (NH2P-40 2D)
- Effects of Eluent pH on HILIC Mode (VG-50 2D)
- Analysis of Thaumatin (SP-825)