LC/MS Analysis of Various Sweeteners (NH2P-40 2D)

Various sweetners were analyzed using Asahipak NH2P-40 2D, a polymer-based amino column, with LC/MS/MS detection. Anion substances can be eluted under alkaline conditions. Furthermore, alkaline conditions promote the deprotonation of hydroxyl groups at the time of ionization. Therefore, alkaline conditions are suitable for high sensitive detection of substances with hydroxyl groups such as saccharides under the negative mode.
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Sample : 5 μL
100 ng/mL each (in H₂O/CH₃CN=1/3)
Dulcin
Sucralose
Acesulfame
Aspartame
Cyclamate
Saccharin
Glucose
Rebaudioside A
Sucrose

Column       : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
Eluent       : (A); 0.1 % NH3 aq./(B); CH3CN
               Isocratic; (B %) 80 %
Flow rate    : 0.2 mL/min
Detector     : ESI-MS (SIM Negative)
Column temp. : 30 °C