超迅速分析用有機溶媒系SEC(GPC)用カラムGPC HK-403を用いて標準ポリスチレンの分析を行いました。RI検出のクロマトグラムでは、プロピルベンゼンとベンゼンが溶媒由来のマイナスピークと重なります。


RI
Sample : 0.5 % each, 5 µL
1. Polystyrene (Mp: 139,000)
2. Polystyrene (Mp: 53,300)
3. Polystyrene (Mp: 21,800)
4. Polystyrene (Mp: 12,000)
5. Polystyrene (Mp: 9,860)
6. Polystyrene (Mp: 6,940)
7. Polystyrene (Mp: 3,180)
8. Polystyrene (Mp: 1,270)
9. Polystyrene (Mp: 580)
10. n-Propylbenzene (MW: 120)
11. Benzene (MW: 78)

UV
Sample : 0.1 % each, 5 µL
1. Polystyrene (Mp: 139,000)
2. Polystyrene (Mp: 53,300)
3. Polystyrene (Mp: 21,800)
4. Polystyrene (Mp: 12,000)
5. Polystyrene (Mp: 9,860)
6. Polystyrene (Mp: 6,940)
7. Polystyrene (Mp: 3,180)
8. Polystyrene (Mp: 1,270)
9. Polystyrene (Mp: 580)
10. n-Propylbenzene (MW: 120)
11. Benzene (MW: 78)

Columns      : Shodex GPC HK-403 (4.6 mm I.D. x 150 mm) x 2
Eluent       : THF
Flow rate    : 1.0 mL/min
Detector     : RI (small cell volume), UV (254 nm) (small cell volume)
Column temp. : 25 ℃