Enoxaparin sodium is a low-molecular-weight heparin with an average molecular weight of about 4,500. It is used as an anticoagulant to inhibit blood coagulation. IC NI-424, an anion analysis column for non-suppressor method, was used to detect sulfate in enoxaparin sodium.


Sample : 30 μL
(A)  USP Enoxaparin sodium RS, 1000 mg/L
(B)  SO₄^2- standard, 2.5 mg/L
1. SO₄^2-
＊ Cl^-

Column       : Shodex IC NI-424 (4.6mm I.D. x 100 mm)
Eluent       : 8 mM 4-Hydroxybenzoic acid + 2.8 mM Bis-Tris + 2 mM Phenylboronic acid
               + 0.005 mM *CyDTA aq.
Flow rate    : 1.0 mL/min
Detector     : Non-suppressed conductivity, 35 ℃
Column temp. : 40 °C

* CyDTA: trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid