Anion Standards (15) (Comparison of NI-424 and I-524A)

The seven main anions can be separated using an anion analysis column for non-suppressor method IC NI-424. The theoretical plate number of the column is more than double that of the existing column IC I-524A.

Features of NI-424 Column

  1. (1)NI-424 column has achieved separation of phosphoric acid ion and fluoride ion which was difficult to sepatate using I-524A.
  2. (2)With NI-424, each peak is sharper, and separation of ions and peak balance have been improved. In particular, improvement has been made in the separation of chloride and nitrite ions making NI-424 column suited to the analysis of a trace amount of nitrite ion contained in high-concentration chloride ion; demand for such analysis is high in the area of environmental analysis.

Sample: 20 µL

  1. 1.H2PO4- 10 mg/L
  2. 2.F- 1 mg/L
  3. 3.Cl- 1 mg/L
  4. 4.NO2- 5 mg/L
  5. 5.Br- 5 mg/L
  6. 6.NO3- 5 mg/L
  7. 7.SO42- 5 mg/L
Column
Shodex IC I-524A (4.6 mm I.D. x 100 mm)
Eluent
2.5 mM Phthalic acid + 2.3 mM Tris(hydroxymethyl)aminomethane aq.
Flow rate
1.2 mL/min
Detector
Non-suppressed conductivity
Column temp.
40 ℃

 

Column
Shodex IC NI-424 (4.6 mm I.D. x 100 mm)
Eluent
8 mM 4-Hydroxybenzoic acid + 2.8 mM Bis-Tris + 2 mM Phenylboronic acid + 0.005 mM *CyDTA aq.
*CyDTA: trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid
Flow rate
1.0 mL/min
Detector
Non-suppressed conductivity
Column temp.
40 ℃

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