IC NI-424 (an anion analysis column for non-suppressor method) has high theoretical plate number (more than 5,000). Carbonate and other anions can be separated well with NI-424.


Sample : 20 μL
1. Acetic acid 20.0 mg/L
2. Formic acid 20.0 mg/L
3. Malic acid 20.0 mg/L
4. Tartaric acid 20.0 mg/L
5. Oxalic acid 20.0 mg/L
6. H₂PO₄^- 20.0 mg/L
7. F^- 1.5 mg/L
8. Cl^- 2.5 mg/L
9. NO₂^- 6.0 mg/L
10. Br^- 10.0 mg/L
11. NO₃^- 10.0 mg/L
12. SO₄^2- 10.0 mg/L

Column       : Shodex IC NI-424 (4.6 mm I.D. x 100 mm)
Eluent       : 8 mM 4-Hydroxybenzoic acid + 2.8 mM Bis-Tris + 2 mM Phenylboronic acid 
               + 0.005 mM *CyDTA aq.
Flow rate    : 1.0 mL/min
Detector     : Non-suppressed conductivity
Column temp. : 40 °C

* CyDTA: trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid