Alkyl sulfonic acids were analyzed using IC NI-424, an anion analysis column for non-suppressor method. It was shown that the longer alkyl chains result with stronger the hydrophobic interaction between the packing material and the sample. With longer alkyl chains, retention time will increase.


Sample : 20 μL
1. Methanesulfonic acid 20 mg/L
2. Ethanesulfonic acid 50 mg/L
3. Propanesulfonic acid 100 mg/L
4. Butanesulfonic acid 100 mg/L
5. Pentanesulfonic acid 300 mg/L
6. Hexanesulfonic acid 200 mg/L
7. Toluenesulfonic acid 200 mg/L

Column       : Shodex IC NI-424 (4.6 mm I.D. x 100 mm)
Eluent       : 8 mM 4-Hydroxybenzoic acid + 3.4 mM Bis-Tris + 2 mM Phenylboronic acid
               + 0.005 mM *CyDTA aq.
Flow rate    : 1.0 mL/min
Detector     : Non-suppressed conductivity
Column temp. : 40 ℃

* CyDTA: trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid