Elution volume of organic acids using a column for anion anlysis, IC NI-424 is shown.
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| Substances | Elution Volume (mL) | Substances | Elution Volume (mL) |
|---|---|---|---|
| Acetic acid | 1.98 | Methacrylic acid | 4.21 |
| Levulinic acid | 2.34 | Malic acid | 4.23 |
| Propionic acid | 2.43 | Malonic acid | 4.83 |
| Glycolic acid | 2.43 | Pimelic acid | 5.20 |
| Lactic acid | 2.68 | n-Valeric acid | 5.85 |
| Pyroglutamic acid | 2.83 | Tartaric acid | 7.64 |
| Formic acid | 2.90 | Trifluoroacetic acid | 7.86 |
| Succinic acid | 3.06 | Oxalic acid | 8.84 |
| Acrylic acid | 3.11 | Fumaric acid | 9.18 |
| Glutaric acid | 3.22 | Citraconic acid | 10.91 |
| n-Butyric acid | 3.44 | Maleic acid | 11.11 |
| Pyruvic acid | 3.50 | cis-Aconitic acid | 19.73 |
| Adipic acid | 3.79 | Phthalic acid | 27.45 |
- Column
- ：Shodex IC NI-424 (4.6 mm I.D. x 100 mm)
- Eluent
- ：8 mM 4-Hydroxybenzoic acid + 2.8 mM Bis-Tris + 2 mM Phenylboronic acid + 0.005 mM *CyDTA aq.
(* trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid) - Flow rate
- ：1.0 mL/min
- Detector.
- ：Non-suppressed conductivity
- Column temp.
- ：40 ℃
Sample Name Index
- Acetic acid
- Acrylic acid
- Adipic acid
- Citraconic acid
- Formic acid
- Fumaric acid
- Glutaric acid
- Glycolic acid
- Lactic acid
- Levulinic acid
- Maleic acid
- Malic acid
- Malonic acid
- Methacrylic acid
- Oxalic acid
- Phthalic acid
- Pimelic acid
- Propionic acid
- Pyroglutamic acid
- Pyruvic acid
- Succinic acid
- Tartaric acid
- Trifluoroacetic acid, TFA
- cis-Aconitic acid
- n-Butyric acid
- n-Valeric acid
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Effects of Sample Load on Column Efficiency (4) (NI-424)
- Linearity of Calibration Curves (NI-424)
- Calibration Curves for Low Concentration F- (NI-424)
- Anion Standards (15) (Comparison of NI-424 and I-524A)
- Anion Standards (16) (NI-424: Phthalic Acid aq. Eluent)
- Anion Standards (17) (Hydrophobic Inorganic Ions) (NI-424)
- Anion Standards (18) (NI-424)
- Tap Water (7) (NI-424)
- River Water (3) (NI-424)
- Rain Water (NI-424)
- Phosphonates (NI-424)
- Effects of pH on Retention Time (NI-424)
- Analysis of Pyrophosphate and Tripolyphosphate (NI-424)
- Carbonate and Other Anions (2) (NI-424)
- Anions and Organic Acids (NI-424)
- Methanesulfonic Acid and Methanedisulfonic Acid (NI-424)
- Analysis of Alkyl Sulfonic Acids (1) (NI-424)
- Anion Analysis in Lithium Salts (1) (NI-424)
- Analysis of Sulfamic Acid and Ammonium Thiocyanate According to Japanese Testing Methods for Fertilizers (NI-424)
- Analysis of Chloride According to Japanese Testing Methods for Fertilizers (NI-424)
- Sulfate Ion in Enoxaparin Sodium (NI-424)