Nucleobases (4) (DE-413)

Six components were completely separated using RSpak DE-413 (a column for reversed phase chromatography).


Sample : 0.01 % each, 5 μL
1. Cytosine
2. Uracil
3. Hypoxanthine
4. Guanine
5. Thymine
6. Adenine


Column       : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : 0.05 M KH2PO4 aq.
Flow rate    : 1.0 mL/min
Detector     : UV (260 nm)
Column temp. : 25 °C

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