Six components were completely separated using RSpak DE-413 (a column for reversed phase chromatography).
Sample : 0.01 % each, 5 μL
1. Cytosine
2. Uracil
3. Hypoxanthine
4. Guanine
5. Thymine
6. Adenine
Column : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm) Eluent : 0.05 M KH2PO4 aq. Flow rate : 1.0 mL/min Detector : UV (260 nm) Column temp. : 25 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Nucleobases and Nucleosides (1) (DE-613)
- Nucleobases and Nucleosides (2) (NN-814)
- Nucleobases, Nucleosides and Nucleotides (GS-320 HQ)
- Nucleobases (1) (GS-320 HQ)
- Guanine and Tryptophan (DM-614, NN-814 and DE-613)
- Adenosine and Derivatives
- "Umami" (GS-320 HQ)
- Nucleosides (1) (GS-320 HQ)
- Adenine Nucleotides in Red Blood Cell
- Purine Bases and Uric Acid Separation (GS-320 HQ)
- Purine Base in Beer (GS-320 HQ)