Adenosine and derivatives were analyzed using Asahipak GS-320 HQ (a multimode column).


Sample : 100 μg/mL each, 20 μL
1. ATP
2. ADP
3. AMP
4. Adenine
5. Adenosine

Column       : Shodex Asahipak GS-320 HQ (7.5 mm I.D. x 300 mm)
Eluent       : 200 mM NaH2PO4 aq./ 200 mM H3PO4 aq.=500/62 
Flow rate    : 1.0 mL/min
Detector     : UV (260 nm)
Column temp. : 30 °C