Guanine and tryptophan were analyzed using three columns for reversed phase chromtography, RSpak DM-614, NN-814 and DE-613.
Sample : 1. Guanine, 2. Tryptophan
Columns : (left); Shodex RSpak DM-G 4A (4.6 mm I.D. x 10 mm) + DM-614 (6.0 mm I.D. x 150 mm) (Center); Shodex RSpak NN-G (6.0 mm I.D. x 50 mm) + NN-814 (8.0 mm I.D. x 250 mm) (Right); Shodex RSpak DE-G 4A (4.6 mm I.D. x 10 mm) + DE-613 (6.0 mm I.D. x 150 mm) Eluent : (Left); 0.3 M K2HPO4 (pH8.6) (Center); 0.1 M NaH2PO4 (pH3.0 adjusted by H3PO4) (Right); 0.23 M K2HPO4 aq./CH3OH=80/20 Flow rate : (Left); 0.8 mL/min, (Center); 1.0 mL/min, (Right); 0.8 mL/min Detector : (Left); UV (278 nm), (Center); RI, (Right); UV (278 nm) Column temp.: (Left); 30 °C, (Center); 50 °C, (Right); 30 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Nucleobases and Nucleosides (1) (DE-613)
- Nucleobases and Nucleosides (2) (NN-814)
- Nucleobases, Nucleosides and Nucleotides (GS-320 HQ)
- Nucleobases (1) (GS-320 HQ)
- Nucleobases (4) (DE-413)
- Adenosine and Derivatives
- "Umami" (GS-320 HQ)
- Nucleosides (1) (GS-320 HQ)
- Adenine Nucleotides in Red Blood Cell
- Purine Bases and Uric Acid Separation (GS-320 HQ)
- Purine Base in Beer (GS-320 HQ)