Asahipak GS-320 HQ (a multimode column) is suitable not only for the separation of nucleobases, nucleosides and nucleotides but also for the separation of samples of their mixtures. GS-320 HQ permits one-time separation by isocratic elution without using gradient elution.


Sample : 20 μL
1. ATP 100 μg/mL
2. ADP 100 μg/mL
3. IMP 100 μg/mL
4. AMP 50 μg/mL
5. GMP 100 μg/mL
6. Inosine 100 μg/mL
7. Adenine 100 μg/mL
8. Hypoxanthine 100 μg/mL
9. Guanine 100 μg/mL
10. Adenosine 100 μg/mL

Column       : Shodex Asahipak GS-320 HQ (7.5 mm I.D. x 300 mm)
Eluent       : 205 mM NaH2PO4 aq./205 mM H3PO4 aq.=300/7
Flow rate    : 0.6 mL/min
Detector     : UV (260 nm)
Column temp. : 30 °C