Asahipak GS-320 HQ is a multimode column and capable of analyzing typical "Umami" (nucleic acids), i.e. IMP, GMP and AMP (nucleotide), and all of their metabolites with simple isocratic elution. The main characteristic of the column is that the nine components(IMP, GMP, AMP and their respective nucleotides, purine bases and pyrimidine bases) are completely separated for approximately 35 minutes and elute in order of nucleotides, nucleosides, purine bases and pyrimidine bases.

Sample : 50 μg/mL each, 20 μL
1. IMP
2. GMP
3. AMP
4. Inosine
5. Hypoxanthine
6. Guanosine
7. Guanine
8. Adenosine
9. Adenine

Column       : Shodex Asahipak GS-320 HQ (7.5 mm I.D. x 300 mm)
Eluent       : 10 mM NaH2PO4 aq./10 mM Na2HPO4 aq.=1000/31
Flow rate    : 1.0 mL/min
Detector     : UV (260 nm)
Column temp. : 40 °C

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