Six components were separated using RSpak NN-814 with pH3.0 phosphate buffer as the eluent. Since cation exchange mode is contributing to the separation, the elution order is different from that of the separation with RSpak DE-613.


Sample : 20 μL
1. Uridine 50 μg/mL
2. Uracil 50 μg/mL
3. Guanosine 100 μg/mL
4. Cytidine 100 μg/mL
5. Adenosine 50 μg/mL
6. Cytosine 50 μg/mL

Column       : Shodex RSpak NN-814 (8.0 mm I.D. x 250 mm)
Eluent       : 100 mM NaH2PO4 aq./100 mM H3PO4 aq.=500/66
Flow rate    : 1.0 mL/min
Detector     : UV (260 nm)
Column temp. : 25 °C