GABA (γ-aminobutyric acid) is a functional ingredient in foods and has blood pressure suppressing and relaxing effects. It is one of the compounds registered as the Japanese functional ingredient in many numbers. In this application, a commercial supplement containing GABA was analyzed by a polymer-based amino column, Asahipak NH2P-50 4E, using its HILIC mode. Supplements, which do not contain many interfering matrixes can be analyzed without using a post-column method. It provided a good linear calibration curve in the concentration range 1 to 1,000 mg/L.
- (1)Take out the content (powder) from a commercial GABA supplement hard capsule.
- (2)Dissolve 5 mg of the removed powder in 25 mL of eluent.
- (3)Filter using a 0.45-µm membrane filter and use it as the injection sample.
Sample: 5 µL
GABA standard solution 1000 mg/L
Commercial supplement
- 1.GABA (γ-Aminobutyric acid)
- Column
- :Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
- Eluent
- :20 mM Sodium phosphate buffer (pH7.0)/CH3CN=30/70
- Flow rate
- :1.0 mL/min
- Detector
- :UV (210 nm)
- Column temp.
- :30 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Amino Acids (2) (NN-814)
- Amino Acids (3) (P-421S)
- Amino Acids (4) (YS-50)
- Amino Acids (5) (YS-50)
- Histidine and 1-Methylhistidine
- Separation of Carcinogenic Tryptophan Pyrolysis Products (ES-502C 7C)
- LC/MS Analysis of Essential Amino Acids (NH2P-40 2D)
- LC/MS Analysis of Citrulline (NH2P-40 2D)
- LC/MS Analysis of GABA (NH2P-40 2D)
- LC/MS Analysis of Tranexamic Acid (NH2P-40 2D)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- Simultaneous Analysis of Amino Acids with LC/MS (VC-50 2D)
- Analysis of Theanine (NH2P-50 4E)
- Analysis of Theanine in Supplement (NH2P-50 4E)
- Effects of Organic Solvent Concentration on HILIC Mode (NH2P-50 4B)