Shodex™ Capture the Essence
Effects of Organic Solvent Concentration on HILIC Mode (NH2P-50 4B)
The effects of organic solvent was analyzed with a polymer-based HILIC column, Asahipak NH2P-50 4B. The analysis was carried under isocratic mode with different organic solvent ratios. The concentration of acetonitrile in the eluent and the retention time (tR) was plotted on a graph. Polar components were retained stronger as the concentration of acetonitrile increased in the HILC mode eluent.
Sample : 10 μL
Inosine 100 μg/mL
Phenylalanine 100 μg/mL
Tyrosine 10 μg/mL
Column : Shodex Asahipak NH2P-50 4B (4.6 mm I.D. x 50 mm) Eluent : 50 mM CH3COOH in CH3CN/50 mM CH3COOH aq. Flow rate : 1.0 mL/min Detector : UV (270 nm) Column temp. : 40 °C
Sample name index
Product name index
Applications
- Amino Acids (2) (NN-814)
- Amino Acids (3) (P-421S)
- Amino Acids (4) (YS-50)
- Amino Acids (5) (YS-50)
- Histidine and 1-Methylhistidine
- Carcinogenic Tryptophan Pyrolysis Products
- LC/MS Analysis of Essential Amino Acids (NH2P-40 2D)
- LC/MS Analysis of Citrulline (NH2P-40 2D)
- LC/MS Analysis of GABA (NH2P-40 2D)
- LC/MS Analysis of Tranexamic Acid (NH2P-40 2D)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- Simultaneous Analysis of Amino Acids with LC/MS (VC-50 2D)
- Analysis of Theanine (NH2P-50 4E)
- Analysis of Theanine in Supplement (NH2P-50 4E)
- Analysis of GABA Supplement (NH2P-50 4E)
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