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Effects of Organic Solvent Concentration on HILIC Mode (NH2P-50 4B)


The effects of organic solvent was analyzed with a polymer-based HILIC column, Asahipak NH2P-50 4B. The analysis was carried under isocratic mode with different organic solvent ratios. The concentration of acetonitrile in the eluent and the retention time (tR) was plotted on a graph. Polar components were retained stronger as the concentration of acetonitrile increased in the HILC mode eluent.

Sample : 10 μL
Inosine 100 μg/mL
Phenylalanine 100 μg/mL
Tyrosine 10 μg/mL

Column       : Shodex Asahipak NH2P-50 4B (4.6 mm I.D. x 50 mm)
Eluent       : 50 mM CH3COOH in CH3CN/50 mM CH3COOH aq.
Flow rate    : 1.0 mL/min
Detector     : UV (270 nm)
Column temp. : 40 °C

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