GABA or γ-aminobutyric acid was analyzed by Asahipak NH2P-40 2D a polymer-based amino column under HILIC mode. Hydrophilic compounds are retained at a high concentration of acetonitrile under HILIC mode conditions. This is advantageous for high sensitivity analysis by LC/MS.
*Sales of Asahipak NH2P-40 2D used in this application is discontinued. Please consider trying Asahipak NH2P-50 2D.
Sample: 5 μL
GABA (γ-Aminobutyric acid) 10 ng/mL (in H2O/CH3CN=25/75)
- Column
- ：Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
- Eluent
- ：20 mM HCOONH4 aq./CH3CN=30/70
- Flow rate
- ：0.2 mL/min
- Detector
- ：ESI-MS (SIM Positive)
- Column temp.
- ：30 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Amino Acids (2) (NN-814)
- Amino Acids (3) (P-421S)
- Amino Acids (4) (YS-50)
- Amino Acids (5) (YS-50)
- Histidine and 1-Methylhistidine
- Separation of Carcinogenic Tryptophan Pyrolysis Products (ES-502C 7C)
- LC/MS Analysis of Essential Amino Acids (NH2P-40 2D)
- LC/MS Analysis of Citrulline (NH2P-40 2D)
- LC/MS Analysis of Tranexamic Acid (NH2P-40 2D)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- Simultaneous Analysis of Amino Acids with LC/MS (VC-50 2D)
- Analysis of Theanine (NH2P-50 4E)
- Analysis of Theanine in Supplement (NH2P-50 4E)
- Analysis of GABA in a Supplement (NH2P-50 4E)
- Effects of Organic Solvent Concentration on HILIC Mode (NH2P-50 4B)