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Simultaneous Analysis of Amino Acids with LC/MS (VC-50 2D)


HILICpak VC-50 2D is a polymer-based amino HILIC column that was used to analyze 20 amino acids. This method does not require the use of derivatization or ion-pair reagents. The result showed basic amino acids were separated well and tend to retain longer by the carboxyl functional group modified on VC-50 2D column packing material. When analyzing acidic amino acids, we recommend HILICpak VG-50 2D and Asahipak NH2P-40 2D.
Refer to:
Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
LC/MS Analysis of Essential Amino Acids (NH2P-40 2D)

Sample : Amino acid standards 0.1μg/mL each (in H2O), 5μL

Aspartic acid, Asp Glutamic acid, Glu Cysteine, Cys Threonine, Thr
Serine, Ser Proline, Pro Tyrosine, Tyr Asparagine, Asn
Methionine, Met Glutamine, Gln Lysine, Lys Alanine, Ala
Glycine, Gly Phenylalanine, Phe Valine, Val Leucine, Leu
Isoleucine, Ile Tryptophan, Trp Histidine, His Arginine, Arg

Column       : Shodex HILICpak VC-50 2D (2.0mmI.D. x 150mm)
Eluent       : H2O/100mM HCOOH aq./CH3CN
               =30/20/50 to 0/90/10 (0 to 5min), 0/90/10 (5 to 30min)
               (Low pressure linear gradient)
Flow rate    : 0.2 mL/min
Detector     : ESI-MS SIM
Column temp. : 30℃

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