A polymer-based amino HILIC column, Asahipak NH2P-40 2D, was used to analyze 20 amino acids that consists proteins. This method does not require the use of delivertization or ion-pair reagents. The analysis takes less than 35 min. The result show that acidic amino acids tend to be retained longer by the amino function group modified on NH2P-40 2D column packed gel.
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Sample: 5 μL
Amino acid standards 5 μg/mL each (in H2O/CH3CN=25/75)
Phenylalanine, Phe | Tyrosine, Tyr | Asparagine, Asn |
Leucine, Leu | Lysine, Lys | Serine, Ser |
Isoleucine, Ile | Alanine, Ala | Cysteine, Cys |
Tryptophan, Trp | Histidine, His | Glutamic acid, Glu |
Methionine, Met | Threonine, Thr | Aspartic acid, Asp |
Proline, Pro | Glycine, Gly | |
Valine, Val | Glutamine, Gln | |
Arginine, Arg |
- Column
- :Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
- Eluent
- :(A); 100 mM HCOONH4 aq.
(B); CH3CN
Linear gradient;
(B %) 75 % (0 to 10 min), 75 % to 50 % (10 to 11 min), 50 % (11 to 35 min) - Flow rate
- :0.2 mL/min
- Detector
- :ESI-MS (SIM)
- Column temp.
- :30 ℃
Sample Name Index
- Alanine, Ala
- Arginine, Arg
- Asparagine, Asn
- Aspartic acid, Asp
- Cysteine, Cys
- Glutamic acid, Glu
- Glutamine, Gln
- Glycine, Gly
- Histidine, His
- Isoleucine, Ile
- Leucine, Leu
- Lysine, Lys
- Methionine, Met
- Phenylalanine, Phe
- Proline, Pro
- Serine, Ser
- Threonine, Thr
- Tryptophan, Trp
- Tyrosine, Tyr
- Valine, Val
Applications
- Amino Acids (2) (NN-814)
- Amino Acids (3) (P-421S)
- Amino Acids (4) (YS-50)
- Amino Acids (5) (YS-50)
- Histidine and 1-Methylhistidine
- Separation of Carcinogenic Tryptophan Pyrolysis Products (ES-502C 7C)
- LC/MS Analysis of Citrulline (NH2P-40 2D)
- LC/MS Analysis of GABA (NH2P-40 2D)
- LC/MS Analysis of Tranexamic Acid (NH2P-40 2D)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- Simultaneous Analysis of Amino Acids with LC/MS (VC-50 2D)
- Analysis of Theanine (NH2P-50 4E)
- Analysis of Theanine in Supplement (NH2P-50 4E)
- Analysis of GABA in a Supplement (NH2P-50 4E)
- Effects of Organic Solvent Concentration on HILIC Mode (NH2P-50 4B)