Three kinds of tetracycline derivative antibiotics were analyzed using Asahipak ODP-40 4D (a column for reversed phase chromatography).
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Sample : 0.1 ppm each, 20 μL
1. Oxytetracycline
2. Tetracycline
3. Chlortetracycline

Column       : Shodex Asahipak ODP-40 4D (4.6 mm I.D. x 150 mm)
Eluent       : Imidazole buffer/Methanol
Detdctor     : Fluorescence (Ex. 380 nm, Em. 520 nm)
Column temp. : 40 °C