Analysis of Carboplatin (NH2P-50 4E)

Carboplatin, a type of anticancer drug, is called a platinum agent because it contains platinum in its structure. A silica-based amino column is used in Assay of Carboplatin listed in the United States Pharmacopeia and the National Formulary (USPNF 2022 Issue 1*). With reference to this USP monograph, carboplatin was analyzed using Asahiak NH2P-50 4E, a polymer-based amino column. We confirmed that NH2P-50 4E was also suitable for the analysis.

*The version at the time of the application acquisition.

Sample: 10 µL

  1. 1.Carboplatin 1 mg/mL
Carboplatin_chromatogram
Column
Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent
CH3CN/H2O=83/17
Flow rate
1.5 mL/min
Detector
UV (230 nm)
Column temp.
25 ℃

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