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Barbiturates (ODP-50 4D)

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Barbiturates, which are widely used as hypnotics, have a maximum UV absorbance at 240nm when an alkaline eluent used. This characteristics make it possible to obtain a highly sensitive analysis for barbiturates using Asahipak ODP-50 4D, a column for reversed phase chromatography.


Sample :
1. Barbital
2. Phenobarbital
3. Hexobarbital
4. Pentobarbital
5. Secobarbital


Column       : Shodex Asahipak ODP-50 4D (4.6mmI.D. x 150mm)
Eluent       : 50mM Phosphate buffer(pH11.0)/CH3OH=75/25
Flow rate    : 0.5mL/min
Detector     : UV(254nm)
Column temp. : 30°C

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