Barbiturates, which are widely used as hypnotics, have a maximum UV absorbance at 240 nm when an alkaline eluent used. This characteristics make it possible to obtain a highly sensitive analysis for barbiturates using Asahipak ODP-50 4D, a column for reversed phase chromatography.


Sample :
1. Barbital
2. Phenobarbital
3. Hexobarbital
4. Pentobarbital
5. Secobarbital

Column       : Shodex Asahipak ODP-50 4D (4.6 mm I.D. x 150 mm)
Eluent       : 50 mM Phosphate buffe r(pH11.0)/CH3OH=75/25
Flow rate    : 0.5 mL/min
Detector     : UV (254 nm)
Column temp. : 30 °C