Statins are a group of drugs used to lower cholesterol level by inhibiting HMG-CoA reductase. Seven statins were simultaneously analyzed by LC/MS method using a polymer-based reversed phase column, ODP2 HP-2B.


Sample : 100 ng/mL each, 5 μL
Pravastatin
Pitavastatin
Fluvastatin
Atorvastatin
Mevastatin
Lovastatin
Simvastatin

Column       : Shodex ODP2 HP-2B (2.0 mm I.D. x 50 mm)
Eluent       : (A); 10 mM Ammonium formate aq.
               (B); CH3CN
               (B %) 10 % (0 min) -> 0 % (3 min) -> 5 % (13 min) -> 5 % (20 min)
Flow rate    : 0.2 mL/min
Detector     : ESI-MS (SIM)
Column temp. : 40 °C