Scopolamine and Atropine

Scopolamine and atropine were analyzed using Asahipak ODP-50 4D (a column for reversed phase chromatography).
Please refer to Analysis of Short Amines without Using Ion Pair Reagent .


Sample :
1. Scopolamine
2. Atropine


Column       : Shodex Asahipak ODP-50 4D (4.6 mm I.D. x 150 mm)
Eluent       : 50 mM Phosphate buffer (pH10)/CH3CN=70/30
Flow rate    : 0.6 mL/min
Detector     : UV (210 nm)
Column temp. : 30 °C
Column : ODS (other manufacturer 4.6 mm I.D. x 150 mm) Eluent : 0.1 % Sodium 1-pentanesulfonate/CH3CN=60/40 Flow rate : 1.4 mL/min Detector : UV (210 nm) Column temp. : 60 °C

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Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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