乙腈在流动相中的作用 (1) (KC-811)

使用RSpak KC-811分析有机酸时，流动相中添加10 %的乙腈，可以缩短疏水性强的有机酸的洗脱时间。

可滚动
Substances Elution Volume (mL)
Eluent (1) Eluent (2)
Oxalic acid   9.28   9.62
Maleic acid   9.91 10.51
α-Ketoglutaric acid 10.39 10.87
Citric acid 10.99 11.71
Isocitric acid 11.05 11.88
2-Ketogluconic acid 11.18 11.52
Glucuronic acid 11.29 11.71
Oxaloacetic acid 11.40 11.77
Pyruvic acid 11.42 11.76
Phosphoric acid 11.43 11.27
Tartaric acid 11.53 12.14
trans-Aconitic acid 11.57 13.11
Galacturonic acid 11.90 12.41
Malonic acid 12.10 12.96
Gluconic acid 12.24 12.78
Malic acid 12.37 13.37
Glyoxylic acid 12.70 13.26
α-Hydroxyglutaric acid 12.71 14.02
Ascorbic acid 12.71 13.88
Succinic acid 13.77 15.79
Itaconic acid 14.08 16.80
Fumaric acid 14.14 17.16
Uric acid 14.99 18.31
Glycolic acid 15.12 16.26
Adipic acid 15.16 20.17
Lactic acid 15.23 16.67
2-Hydroxyisobutyric acid 15.26 17.05
Mesaconic acid 15.42 20.47
β-Hydroxypropionic acid 15.61 17.18
Levulinic acid 16.44 20.27
Formic acid 16.59 17.86
Acetic acid 17.32 19.41
Pyroglutamic acid 18.02 20.99
Propionic acid 18.82 22.54
Isobutyric acid 20.13 25.59
n-Butyric acid 20.87 27.61
Isovaleric acid 22.16 32.28
HCO3- 23.36 24.50
n-Valeric acid 24.64 38.89
Columns
：Shodex RSpak KC-811 (8.0 mm I.D. x 300 mm) x 2
Eluent
：(1) ; 3 mM HClO4 aq./CH3CN=90/10
(2) ; 3 mM HClO4 aq.
Flow rate
：1.0 mL/min
Column temp.
：50 ℃

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