流动相浓度对有机酸洗脱体积的影响 (KC-811)

根据流动相的pH值、分析温度的不同，有机酸的离解状态会发生改变，使用RSpak KC-811分析有机酸会影响分离模式。

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Substances Elution Volume (mL)
Eluent (1) Eluent (2) Eluent (3) Eluent (4)
Oxalic acid   9.27   9.41   9.62   9.72
Maleic acid   9.70 10.15 10.51 11.12
α-Ketoglutaric acid   9.96 10.44 10.87 11.37
Oxaloacetic acid 10.48 11.22 11.77 12.50
Pyruvic acid 10.51 11.24 11.76 12.48
2-Ketogluconic acid 10.59 11.13 11.52 11.73
Phosphoric acid 10.61 10.95 11.27 11.58
Citric acid 10.84 11.32 11.71 11.81
Tartaric acid 11.06 11.66 12.14 12.31
Isocitric acid 11.10 11.54 11.88 11.89
Glucuronic acid 11.16 11.44 11.71 11.76
trans-Aconitic acid 11.47 12.43 13.11 13.48
Malonic acid 11.54 12.36 12.96 13.40
Galacturonic acid 11.83 12.12 12.41 12.39
Gluconic acid 12.29 12.53 12.78 12.48
Glyoxylic acid 12.35 12.86 13.26 13.86
Malic acid 12.50 12.99 13.37 13.42
α-Hydroxyglutaric acid 13.47 13.91 14.02 14.15
Ascorbic acid 13.53 13.69 13.88 13.80
Fumaric acid 14.47 16.09 17.16 17.75
Succinic acid 15.30 15.52 15.79 15.69
Glycolic acid 15.60 15.92 16.26 16.22
Itaconic acid 15.92 16.42 16.80 16.79
Lactic acid 16.02 16.39 16.67 16.68
2-Hydroxyisobutyric acid 16.52 16.82 17.05 17.05
Adipic acid 16.65 19.84 20.17 20.01
β-Hydroxypropionic acid 16.94 17.04 17.18 17.12
Formic acid 17.18 17.55 17.86 17.92
Mesaconic acid 17.20 19.20 20.47 21.06
Uric acid 17.89 17.93 18.31 18.00
Pyroglutamic acid 18.59 20.25 20.99 21.65
Acetic acid 19.19 19.29 19.41 19.36
Levulinic acid 20.01 20.19 20.27 20.23
Propionic acid 22.33 22.43 22.54 22.49
HCO3- 24.72 24.72 24.50 24.68
Isobutyric acid 25.35 25.47 25.59 25.55
n-Butyric acid 27.32 27.46 27.61 27.57
Isovaleric acid 31.89 32.07 32.28 32.20
n-Valeric acid 38.36 38.57 38.89 38.75
Columns
：Shodex RSpak KC-811 (8.0 mm I.D. x 300 mm) x 2
Eluent
：(1) ; 1 mM HClO4 aq.
(2) ; 2 mM HClO4 aq.
(3) ; 3 mM HClO4 aq.
(4) ; 5 mM HClO4 aq.
Flow rate
：1.0 mL/min
Column temp.
：50 ℃

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