Coenzyme Q₁₀, also known as ubiquinone Q₁₀ (or ubiquinone), is a fat-soluble substance found in foods such as meat and seafood. It is expected to have antioxidant and anti-aging effects and is also known as a vitamin-like substance. In this application, we analyzed a commercially available supplement (soft capsule) using a silica-based reversed-phase chromatography column, Silica C18M 4D. C18M 4D was feasible analyzing coenzyme Q₁₀ without having interference from other components. In addition, obtained calibration curve prepared using standard samples showed a highly linear calibration curve with a coefficient of determination (R²) 0.9991, in the concentration range of 10 to 1000 mg/L.

Sample pretreatment

1. 1.Remove the powder contents of the supplement (soft capsule).
2. 2.Add 4 mL of eluent to 5 mg of the sample. Apply sonication for 10 minutes, and then heat at 40℃ for 2 minutes to dissolve the sample completely.
3. 3.Let it cool to room temperature and make up to 5 mL with eluent.
4. 4.Filter with 0.45 µm filter and use it as a sample.

Sample: 5 μL
(Sample solution)
(Standard solution) 1000 mg/L in eluent

1. 1.Coenzyme Q₁₀