Unsaturated fatty acids are expected to prevent arteriosclerosis and thrombosis, and improve visual function and allergy symptoms. In this application, three kinds of Omega-3 fatty acids and two kinds of Omega-6 fatty acids were analyzed using Asahipak ODP-50 4D, a polymer-based reversed phase chromatography column.
Sample : 5 µL
0.002 % each (in Ethanol)
1. EPA (Eicosapentaenoic acid)
2. α-Linolenic acid
3. DHA (Docosahexaenoic acid)
4. Arachidonic acid
5. Linoleic acid
Column : Shodex Asahipak ODP-50 4D (4.6 mm I.D. x 150 mm) Eluent : 0.1 % H3PO4 in (H2O/CH3CN=30/70) Flow rate : 1.0 mL/min Detector : UV (215 nm) Column temp. : 40 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Leukotrienes (ODP-50 6D)
- Leukotrienes Produced by Polymorphonuclear Leukocyte of Rat (ODP-50 6D)
- Hydroxyeicosatetraenoic Acid Produced by Polymorphonuclear Leukocyte of Rat (ODP-50 6D)
- Hydroxyeicosatetraenoic Acids (ODP-50 6D)
- Plasma Lipoproteins (1) (SB-805 HQ)
- Glycerides (1) (Chloroform Eluent) (GF-310 HQ)
- Glycerides (2) (THF Eluent) (GF-310 HQ)
- Glycerides (3) (DMF Eluent) (GF-310 HQ)
- Triglycerides (GF-310 HQ)
- Comparison of Triglycerides Separation
- Fatty Acids (6) (KF-402HQ)
- Analysis of Fatty Acids with Corona CAD (DS-613)
- Fatty Acid Methyl Esters (DS-413)
- Analysis of Unsaturated Fatty Acids in Supplements (ODP-50 4D)
- Analysis of α-Linolenic Acid in Edible Blended Oil (ODP-50 4D)
- Analysis of Coenzyme Q10 in a Supplement (C18M 4D)