Unsaturated Fatty Acids (ODP-50 4D)

Unsaturated fatty acids are expected to prevent arteriosclerosis and thrombosis, and improve visual function and allergy symptoms. In this application, three kinds of Omega-3 fatty acids and two kinds of Omega-6 fatty acids were analyzed using Asahipak ODP-50 4D, a polymer-based reversed phase chromatography column.


Sample : 5 µL
0.002 % each (in Ethanol)
1. EPA (Eicosapentaenoic acid)
2. α-Linolenic acid
3. DHA (Docosahexaenoic acid)
4. Arachidonic acid
5. Linoleic acid


Column       : Shodex Asahipak ODP-50 4D (4.6 mm I.D. x 150 mm)
Eluent       : 0.1 % H3PO4 in (H2O/CH3CN=30/70)
Flow rate    : 1.0 mL/min
Detector     : UV (215 nm)
Column temp. : 40 °C

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