Hydroxyeicosatetraenoic Acids (ODP-50 6D)

HETEs (hydroxyeicosatetraenoic acids) were analyzed using Asahipak ODP-50 6D ( a column for reversed phase chromatography ).


Sample : Hydroxyeicosatetraenoic acids
1. 15-HETE
2. 12-HETE
3. 5-HETE


Column       : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent       : H2O/CH3COOH/CH3CN/CH3OH=50/0.1/50/10
Flow rate    : 1.0 mL/min
Detector     : UV (235 nm)
Column temp. : 30 °C

Courtesy of Dr. Oishi and Dr. Yamaki, Kitazato University

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