Leukotrienes Produced by Polymorphonuclear Leukocyte of Rat (ODP-50 6D)

Leukotrienes produced by polymorphonuclear leukocyte of rat were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography).


Sample : Leukotrienes


Column       : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent       : 0.2 M CH3COONa buffer (pH4.5 adjusted with CH3COOH)/CH3CN/CH3OH=50/25/25
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C

Courtesy of Dr. Oishi and Dr. Yamaki, Kitazato University

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