Polyamine and acetylated derivatives were analyzed using Asahipak ODP-50 4E (a column for reversed phase chromatography) with alkaline eluent.
Sample :
1. Putrescine
2. Acetylputrescine
3. Cadaverine
4. Spermidine
5. Acetylcadaverine
6. N1-Acetylspermidine
7. N8-Acetylspermidine
8. Spermine
9. Acetylspermine
Column : Shodex Asahipak ODP-50 4E (4.6 mm I.D. x 250 mm) Eluent : 200 mM Sodium phosphate (pH7.7) + 2.3 mM Sodium 1-octanesulfonate Flow rate : 0.5 mL/min Detector : Fluoresence (Ex. 345 nm, Em. 455 nm) (OPA post-column reaction) Column temp. : Room temp.
Courtesy of Dr. Kamei, The Tokyo Metropolitan Institute od Medical Science
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- HVA and VMA Standards (ES-502N 7C)
- Analysis of Polyamines by On-Column Derivertization (ODP-50 4D)
- Analysis of Histamine by On-Column Derivertization (ODP-50 4E)
- Kojic Acid (DE-413)
- Analysis of Metabolites from Nitrogen Containing Compounds (YS-50)
- LC/MS/MS Analysis of Creatine and Related Substances (NH2P-40 2D)
- Analysis of Choline and Acetylcholine (YS-50)
- LC/MS Analysis of Choline and Acetylcholine (VC-50 2D)