Kojic acid was analyzed using RSpak DE-413 (a column for reversed phase chromatography). Kojic acid is a by-product in the production of koji which is malted rice and used for one of the raw materials of sake, Japanese rice wine. Kojic acid is an antibacterial metabolic microorganism.


Sample :
1. Kojic acid
(5-Hydroxy-2-(hydroxymethyl)-4H-pyran-4-one)

Column       : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : 10 mM H3PO4 aq.
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 40 °C