Urea, creatine, creatinine were separated using ion (cation) chromatography column, IC YS-50. Urea and creatinine are known as final metabolites of nitrogen containing compounds.


Sample : 10 μL
1. Urea 0.06 %
2. Creatine 0.001 %
3. Creatinine 0.001 %

Column       : Shodex IC YS-50 (4.6 mm I.D. x 125 mm)
Eluent       : 10 mM H3PO4 aq.
Flow rate    : 1.0 mL/min
Detector     : UV (210 nm)
Column temp. : 40 °C