Analysis of Choline and Acetylcholine (YS-50)

Choline and acetylcholine were analyzed using IC YS-50, a cation analysis column, under cation exchange mode. If high sensitive analysis is required, LC/MS/MS analysis using HILICpak VC-50 2D, a polymer-based HILIC mode column, is recommended. When VC-50 2D is used, the elution order of a sample is reversed when compared to HILIC mode.
Refer to: LC/MS Analysis of Choline and Acetylcholine (VC-50 2D)


Sample : 20 µL
10 µg/mL each (in H2O)
1. Choline
2. Acetylcholine


Column       : Shodex IC YS-50 (4.6 mm I.D. x 125 mm)
Eluent       : 10 mM H3PO4 aq. 
Flow rate    : 1.0 mL/min
Detector     : Non-suppressed conductivity, UV (210 nm)
Column temp. : 40 °C

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