Shodex™ Capture the Essence
LC/MS Analysis of Choline and Acetylcholine (VC-50 2D)
Choline and acetylcholine were analyzed by LC/MS method using HILICpak VC-50 2D, a polymer-based HILIC column. The calibration curves of choline and acetylcholine show good linearity respectively in the range from 1 to 100mM.
Sample : 5μL
5nM each (in H2O)
1. Acetylcholine
2. Choline
Column : Shodex HILICpak VC-50 2D (2.0mm I.D. x 150mm) Eluent : 200mM HCOOH aq./CH3CN=40/60 Flow rate : 0.3mL/min Detector : ESI-MS/MS (MRM Positive) Column temp. : 40°C
Sample name index
Product name index
Applications
- HVA and VMA Standards
- Analysis of VMA and Creatinine using Column Switching Method
- HVA and VMA in Urine
- Analysis of Uric Acid and Creatinine using Column Switching Method
- Polyamine and Acetylated Derivatives
- Analysis of Polyamines by On-Column Derivertization
- Analysis of Histamine by On-Column Derivertization
- Kojic Acid
- Analysis of Metabolites from Nitrogen Containing Compounds
- LC/MS/MS Analysis of Creatine and Related Substances (NH2P-40 2D)
- Analysis of Choline and Acetylcholine
- Analysis of Choline and Acetylcholine (YS-50)
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