LC/MS Analysis of Choline and Acetylcholine (VC-50 2D)

Choline and acetylcholine were analyzed by LC/MS method using HILICpak VC-50 2D, a polymer-based HILIC column. The calibration curves of choline and acetylcholine show good linearity respectively in the range from 1 to 100 mM.

Sample: 5 µL
5 nM each (in H2O)

  1. 1.Acetylcholine
  2. 2.Choline
Column
Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm)
Eluent
200 mM HCOOH aq./CH3CN=40/60
Flow rate
0.3 mL/min
Detector
ESI-MS/MS (MRM Positive)
Column temp.
40 ℃

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