Choline and acetylcholine were analyzed by LC/MS method using HILICpak VC-50 2D, a polymer-based HILIC column. The calibration curves of choline and acetylcholine show good linearity respectively in the range from 1 to 100 mM.
Sample: 5 µL
5 nM each (in H2O)
- 1.Acetylcholine
- 2.Choline
- Column
- ：Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm)
- Eluent
- ：200 mM HCOOH aq./CH3CN=40/60
- Flow rate
- ：0.3 mL/min
- Detector
- ：ESI-MS/MS (MRM Positive)
- Column temp.
- ：40 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- HVA and VMA Standards (ES-502N 7C)
- Polyamine and Acetylated Derivatives (ODP-50 4E)
- Analysis of Polyamines by On-Column Derivertization (ODP-50 4D)
- Analysis of Histamine by On-Column Derivertization (ODP-50 4E)
- Kojic Acid (DE-413)
- Analysis of Metabolites from Nitrogen Containing Compounds (YS-50)
- LC/MS/MS Analysis of Creatine and Related Substances (NH2P-40 2D)
- Analysis of Choline and Acetylcholine (YS-50)