Analysis of Histamine by On-Column Derivertization (ODP-50 4E)

Histamine was analyzed by on-column derivertization using Asahipak ODP-50 4E . When a stimulation, enough to cause inflammation, is applied to mast cells, they release histamine and other physiologically active substances as a result of their cell degradation. The analysis of histamine is widely performed using mast cells by bioassay to assess their inflammation and/or allergy.
This chromatograms show analysis of histamine released during the degradation induced by the compounds 48/80(A), mastobaran (B) and somatostatin (C). Histamine analysis is possible without the interference of coexisting substances.
Refer to Instruments for On-Column Derivetization


Sample : Histamine released from rat peritoneal mast cell inducted by (A), (B) and (C)


Column       : Shodex Asahipak ODP-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : CH3CN - 50 mM Sodium tetraborate(18:82,v/v)
               containing 1 mM OPA(o-Phthalaldehyde) and 1 mM NAC(N-Acetyl-L-cysteine)
Flow rate    : 0.5 mL/min
Detector     : Fluorescence (Ex. 340 nm, Em. 450 nm)
Column temp. : 40 °C
K.Saito, M.Horie, N.Nose,K.Nakagomi and H.Nakazawa, J.Chromatogr., 595,163(1992)

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