Histamine was analyzed by on-column derivertization using Asahipak ODP-50 4E . When a stimulation, enough to cause inflammation, is applied to mast cells, they release histamine and other physiologically active substances as a result of their cell degradation. The analysis of histamine is widely performed using mast cells by bioassay to assess their inflammation and/or allergy.
This chromatograms show analysis of histamine released during the degradation induced by the compounds 48/80(A), mastobaran (B) and somatostatin (C). Histamine analysis is possible without the interference of coexisting substances.
Refer to Instruments for On-Column Derivetization
Sample : Histamine released from rat peritoneal mast cell inducted by (A), (B) and (C)
Column : Shodex Asahipak ODP-50 4E (4.6 mm I.D. x 250 mm) Eluent : CH3CN - 50 mM Sodium tetraborate(18:82,v/v) containing 1 mM OPA(o-Phthalaldehyde) and 1 mM NAC(N-Acetyl-L-cysteine) Flow rate : 0.5 mL/min Detector : Fluorescence (Ex. 340 nm, Em. 450 nm) Column temp. : 40 °CK.Saito, M.Horie, N.Nose,K.Nakagomi and H.Nakazawa, J.Chromatogr., 595,163(1992)
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Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
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Applications
- HVA and VMA Standards (ES-502N 7C)
- Polyamine and Acetylated Derivatives (ODP-50 4E)
- Analysis of Polyamines by On-Column Derivertization (ODP-50 4D)
- Kojic Acid (DE-413)
- Analysis of Metabolites from Nitrogen Containing Compounds (YS-50)
- LC/MS/MS Analysis of Creatine and Related Substances (NH2P-40 2D)
- Analysis of Choline and Acetylcholine (YS-50)
- LC/MS Analysis of Choline and Acetylcholine (VC-50 2D)