Oligogalacturonic acids were separated using HILICpak VN-50 4D, a polymer-based HILIC column. Since galacturonic acid, which is a constituent sugar, has a carboxyl group, it is necessary to keep the eluent under acidic conditions to suppress dissociation. Using a gradient condition of 1 % formic acid aqueous solution and acetonitrile, oligogalacturonic acids were detected with an evaporative light scattering detector (ELSD).  Sharp peaks were obtained and it was possible to confirm up to dp 11.

Sample: 10 μL
Oligogalacturonic acids 5 mg/mL (in 50 % CH₃CN)

1. 1.Galacturonic acid                           7.Oligogalacturonic acid heptamer
2. 2.Oligogalacturonic acid dimer           8.Oligogalacturonic acid octamer
3. 3.Oligogalacturonic acid trimer          9.Oligogalacturonic acid nonamer
4. 4.Oligogalacturonic acid tetramer     10.Oligogalacturonic acid decamer
5. 5.Oligogalacturonic acid pentamer    11.Oligogalacturonic acid undecamer
6. 6.Oligogalacturonic acid hexamer