Analysis of Oligogalacturonic Acids (VN-50 4D)

Oligogalacturonic acids were separated using HILICpak VN-50 4D, a polymer-based HILIC column. Since galacturonic acid, which is a constituent sugar, has a carboxyl group, it is necessary to keep the eluent under acidic conditions to suppress dissociation. Using a gradient condition of 1 % formic acid aqueous solution and acetonitrile, oligogalacturonic acids were detected with an evaporative light scattering detector (ELSD).  Sharp peaks were obtained and it was possible to confirm up to dp 11.

Sample: 10 μL
Oligogalacturonic acids 5 mg/mL (in 50 % CH3CN)

  1. 1.Galacturonic acid                           7.Oligogalacturonic acid heptamer
  2. 2.Oligogalacturonic acid dimer           8.Oligogalacturonic acid octamer
  3. 3.Oligogalacturonic acid trimer          9.Oligogalacturonic acid nonamer
  4. 4.Oligogalacturonic acid tetramer     10.Oligogalacturonic acid decamer
  5. 5.Oligogalacturonic acid pentamer    11.Oligogalacturonic acid undecamer
  6. 6.Oligogalacturonic acid hexamer
Shodex HILICpak VN-50 4D (4.6 mm I.D. x 150 mm)
(A); 1 % HCOOH aq., (B); CH3CN
Linear gradient; (B %) 55 to 35 % (0 to 10 min), 35% (10 to 15 min)
Flow rate
0.6 mL/min
Column temp.
40 ℃

Sample Name Index

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

Product Name Index