Glucose can be anearobically decomposed into lactic acid and ethanol.This decomposing process is called as glucolytic pathway. In this pathway, some products such as glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-bisphosphate, etc. are produced as intermediary products. Here, fructose-6-phosphate and fructose-1,6-bisphosphate are analyzed using Asahipak NH2P-50 4E by ion-exchange mode.


Sample : 5 mg/mL each, 20 μL
1. Fructose-6-phosphate
2. Fructose-1,6-bisphosphate

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)				
Eluent       : 300 mM Sodium phosphate buffer (pH4.4)						
Flow rate    : 1.0 mL/min						
Detector     : RI						
Column temp. : 40 °C