Galacturonic acid is one of uronic acids and exists as a one of components of bacterial polysaccharides such as pectin. This chromatomgram shows an anlysis of galacturonic acid oligomers using an Asahipak NH2P-50 4E column with phosphate buffer as the eluent. Oligogalactronic acids, from monomer to hexamer, are separated.


Sample : Oligogalacturonic acids
(Courtesy of Dr. Yoshino, Kyoto Women's University)
1. Galacturonic acid
2. Oligogalacturonic acid dimer
3. Oligogalacturonic acid trimer
4. Oligogalacturonic acid tetramer
5. Oligogalacturonic acid pentamer
6. Oligogalacturonic acid hexamer

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : 0.3 M Sodium phosphate buffer (pH4.4)
Flow rate    : 1.0 mL/min
Detector     : UV (210 nm)
Column temp. : 40 °C