Phytic acid and Sodium phytic acid were analyzed using 2 columns of Asahipak GS-220 HQ. Phytic acid was eluted near exclusion limit of GS-220 HQ.

Sample : 20 μL
A) Phytic acid sodium salt
B) Phytic acid

1. Phytic acid
2. Na+
3. HClO4- vacant peak

Columns      : Shodex Asahipak GS-220 HQ (7.5 mm I.D. x 300 mm) x 2
Eluent       : 10 mM HClO4 aq.
Flow rate    : 0.6 mL/min
Detector     : RI
Column temp. : 40 °C

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