Separation of N-acetylneuraminic Acid and N-glycolyneuraminic Acid (KC-811)

N-acetylneuraminic acid and N-glycolyneuraminic acid, family of sialic acid, were separated using organic-acids-analysis column, RSpak KC-811.


Sample : 0.1 % each, 40 μL
1. N-Glycolylneuraminic acid
2. N-Acetylneuraminic acid


Column       : Shodex RSpak KC-811 (8.0 mm I.D. x 300 mm)
Eluent       : 1 % H3P04 aq.
Flow rate    : 1.0 mL/min
Detector     : UV (210 nm)
Column temp. : 40 °C

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