Phytic acid is a phosphoric acid ester of myo-inositol, in which six hydroxyl groups are replaced phosphate group, and it is also called myo-inositol hexaphosphate (IP6). In this application, phytic acid, IP5 (inositol pentaphosphate) and IP4 (inositol tetraphosphate) were simultaneously analyzed using HILICpak VG-50 2D, a polymer-based amino column with LC/MS detection. Alkaline conditions are suitable for high sensitive detection.
Sample : 5 μL
11,000-fold diluted Phytic acid (ca.50 % in Water, ca.1.1 mol/L, Tokyo Chemical Industry Co., Ltd. )
1. Phosphoric acid
2. Phytic acid (IP6)
Column : Shodex HILICpak VG-50 2D (2.0 mm I.D. x 150 mm) Eluent : 0.1 M Ammonium carbonate buffer (pH10.0)/CH3CN=60/40 Flow rate : 0.2 mL/min Detector : ESI-MS (SIM Negative) Column temp. : 40 °C
Sample Name Index
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