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LC/MS Analysis of Inositol Phosphates (VG-50 2D)


Phytic acid is a phosphoric acid ester of myo-inositol, in which six hydroxyl groups are replaced phosphate group, and it is also called myo-inositol hexaphosphate (IP6). In this application, phytic acid, IP5 (inositol pentaphosphate) and IP4 (inositol tetraphosphate) were simultaneously analyzed using HILICpak VG-50 2D, a polymer-based amino column with LC/MS detection. Alkaline conditions are suitable for high sensitive detection.

Sample : 5μL
11,000-fold diluted Phytic acid (ca.50% in Water, ca.1.1mol/L, Tokyo Chemical Industry Co., Ltd. )
1. Phosphoric acid
2. Phytic acid (IP6)
3. IP5
4. IP4

Column       : Shodex HILICpak VG-50 2D (2.0mm I.D. x 150mm) 
Eluent       : 0.1M Ammonium carbonate buffer (pH10.0)/CH3CN=60/40
Flow rate    : 0.2mL/min
Detector     : ESI-MS (SIM Negative)
Column temp. : 40°C

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